Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
Identifieur interne : 002A43 ( Main/Exploration ); précédent : 002A42; suivant : 002A44Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
Auteurs : Jianjun Guo [États-Unis] ; Jennifer L. Morrell-Falvey ; Jessy L. Labbé ; Wellington Muchero ; Udaya C. Kalluri ; Gerald A. Tuskan ; Jin-Gui ChenSource :
- PloS one [ 1932-6203 ] ; 2012.
Descripteurs français
- KwdFr :
- Cartographie d'interactions entre protéines (MeSH), Cellules du mésophylle (cytologie), Cellules du mésophylle (effets des médicaments et des substances chimiques), Cellules du mésophylle (métabolisme), Expression des gènes (MeSH), Facteur de croissance végétal (pharmacologie), Facteurs temps (MeSH), Gènes de plante (génétique), Marqueurs biologiques (métabolisme), Milieux de culture (composition chimique), Organites (effets des médicaments et des substances chimiques), Organites (métabolisme), Populus (cytologie), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Protoplastes (cytologie), Protoplastes (effets des médicaments et des substances chimiques), Protoplastes (métabolisme), Séparation cellulaire (méthodes), Transduction du signal (effets des médicaments et des substances chimiques), Transport des protéines (effets des médicaments et des substances chimiques).
- MESH :
- composition chimique : Milieux de culture.
- cytologie : Cellules du mésophylle, Populus, Protoplastes.
- effets des médicaments et des substances chimiques : Cellules du mésophylle, Organites, Populus, Protoplastes, Transduction du signal, Transport des protéines.
- génétique : Gènes de plante, Populus.
- métabolisme : Cellules du mésophylle, Marqueurs biologiques, Organites, Protoplastes.
- méthodes : Séparation cellulaire.
- pharmacologie : Facteur de croissance végétal.
- Cartographie d'interactions entre protéines, Expression des gènes, Facteurs temps.
English descriptors
- KwdEn :
- Biomarkers (metabolism), Cell Separation (methods), Culture Media (chemistry), Gene Expression (MeSH), Genes, Plant (genetics), Mesophyll Cells (cytology), Mesophyll Cells (drug effects), Mesophyll Cells (metabolism), Organelles (drug effects), Organelles (metabolism), Plant Growth Regulators (pharmacology), Populus (cytology), Populus (drug effects), Populus (genetics), Protein Interaction Mapping (MeSH), Protein Transport (drug effects), Protoplasts (cytology), Protoplasts (drug effects), Protoplasts (metabolism), Signal Transduction (drug effects), Time Factors (MeSH).
- MESH :
- chemical , chemistry : Culture Media.
- chemical , metabolism : Biomarkers.
- cytology : Mesophyll Cells, Populus, Protoplasts.
- drug effects : Mesophyll Cells, Organelles, Populus, Protein Transport, Protoplasts, Signal Transduction.
- genetics : Genes, Plant, Populus.
- metabolism : Mesophyll Cells, Organelles, Protoplasts.
- methods : Cell Separation.
- chemical , pharmacology : Plant Growth Regulators.
- Gene Expression, Protein Interaction Mapping, Time Factors.
Abstract
BACKGROUND
Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.
METHODOLOGY/PRINCIPAL FINDINGS
We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts are able to respond to hormonal stimuli and that a series of organelle markers are correctly localized in the Populus protoplasts. Furthermore, we showed that the Populus protoplast transient expression system is suitable for studying protein-protein interaction, gene activation, and cellular signaling events.
CONCLUSIONS/SIGNIFICANCE
This study established a method for efficient isolation of protoplasts from Populus leaf and demonstrated the efficacy of using Populus protoplast transient expression assays as an in vivo system to characterize genes and pathways.
DOI: 10.1371/journal.pone.0044908
PubMed: 23028673
PubMed Central: PMC3441479
Affiliations:
Links toward previous steps (curation, corpus...)
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temps</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.</p></div><div type="abstract" xml:lang="en"><p><b>METHODOLOGY/PRINCIPAL FINDINGS</b></p><p>We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts are able to respond to hormonal stimuli and that a series of organelle markers are correctly localized in the Populus protoplasts. Furthermore, we showed that the Populus protoplast transient expression system is suitable for studying protein-protein interaction, gene activation, and cellular signaling events.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS/SIGNIFICANCE</b></p><p>This study established a method for efficient isolation of protoplasts from Populus leaf and demonstrated the efficacy of using Populus protoplast transient expression assays as an in vivo system to characterize genes and pathways.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23028673</PMID><DateCompleted><Year>2013</Year><Month>03</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>9</Issue><PubDate><Year>2012</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.</ArticleTitle><Pagination><MedlinePgn>e44908</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0044908</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.</AbstractText><AbstractText Label="METHODOLOGY/PRINCIPAL FINDINGS" NlmCategory="RESULTS">We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts are able to respond to hormonal stimuli and that a series of organelle markers are correctly localized in the Populus protoplasts. Furthermore, we showed that the Populus protoplast transient expression system is suitable for studying protein-protein interaction, gene activation, and cellular signaling events.</AbstractText><AbstractText Label="CONCLUSIONS/SIGNIFICANCE" NlmCategory="CONCLUSIONS">This study established a method for efficient isolation of protoplasts from Populus leaf and demonstrated the efficacy of using Populus protoplast transient expression assays as an in vivo system to characterize genes and pathways.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Jianjun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morrell-Falvey</LastName><ForeName>Jennifer L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Labbé</LastName><ForeName>Jessy L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Muchero</LastName><ForeName>Wellington</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Kalluri</LastName><ForeName>Udaya C</ForeName><Initials>UC</Initials></Author><Author ValidYN="Y"><LastName>Tuskan</LastName><ForeName>Gerald A</ForeName><Initials>GA</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jin-Gui</ForeName><Initials>JG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS 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sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><name sortKey="Labbe, Jessy L" sort="Labbe, Jessy L" uniqKey="Labbe J" first="Jessy L" last="Labbé">Jessy L. Labbé</name><name sortKey="Morrell Falvey, Jennifer L" sort="Morrell Falvey, Jennifer L" uniqKey="Morrell Falvey J" first="Jennifer L" last="Morrell-Falvey">Jennifer L. Morrell-Falvey</name><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name></noCountry><country name="États-Unis"><region name="Tennessee"><name sortKey="Guo, Jianjun" sort="Guo, Jianjun" uniqKey="Guo J" first="Jianjun" last="Guo">Jianjun Guo</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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